RNA

Part:BBa_K4409002:Design

Designed by: Kexin Fei   Group: iGEM22_Worldshaper-HZ   (2022-09-20)


guide of igRNA (hsa_circ_0001982)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed to be a guide sequence for the igRNA because dsDNA of hsa_circ_0001982 (BBa_K4409008) is a very good candidate for the substrate dsDNA used in the CRISPR-Cas12 system of our project. Substrate dsDNA is used to activate the Cas12 enzyme. A large amount of dsDNA is needed in the system to achieve sensitive signals. hsa_circ_0001982 dsDNA can be obtained easily and abundantly when our detection tool for hsa_circ_0001785 and hsa_circ_0001982 biomarkers in blood and tissues is applied clinically, because blood samples tested positive by our tool are likely to be further analyzed by amplifying hsa_circ_0001785 or hsa_circ_0001982 for other tests such as gene sequencing. To form an igRNA, this part can be coupled with our trigger-RNA sensor (BBa_K4409003) which contains a loop sequence complementary to it, so that the loop sequence can block this part when no trigger is in the system.


Source

This part is based on the complement sequence of hsa_circ_0001982 sequence which is retrieved from circBank database.

References